论文【WT1肽疫苗诱导WT1（Wilms'肿瘤基因）特异性细胞毒性T淋巴细胞及其导致的癌症消退】
Induction of WT1 (Wilms' tumor gene)-specific cytotoxic T lymphocytes by WT1 peptide vaccine and the resultant cancer regression.
（PMID：15365188 PMCID：PMC518848）
该WT1肽的I期临床研究得到了大阪大学医学院和广岛红十字会 Hiroshima Red Cross 和 Atomic Bomb Survivor Hospital医院的批准
The WT1 peptide-based phase I clinical study was approved by the Ethical Review Boards of the Faculty of Medicine, Osaka University and Hiroshima Red Cross and Atomic Bomb Survivor Hospital.
结果
在三名患有肺癌的患者中，观察到由WT1疫苗接种引起的肿瘤标志物的减少。
In three patients with lung cancer (patients 3, 6, and 12), a decrease in tumor markers caused by WT1 vaccination was observed.
在患者3中，其肺癌对化学疗法和放射疗法完全有抗性，在WT1疫苗接种后立即开始降低肿瘤胚抗原水平从2,048ng / ml开始降低，并且在第七次WT1疫苗接种后达到805ng / ml。在第二次WT1疫苗接种后，肺野中的肿瘤块消退。
In patient 3, whose lung cancer was completely resistant to chemotherapy and radiotherapy, chorio-embryonic antigen levels began to decrease from 2,048 ng/ml immediately after the WT1 vaccination and reached 805 ng/ml after the seventh WT1 vaccination. The tumor masses in the lung fields regressed after the second WT1 vaccination (34).
在患者6中，SLX，在手术后已恢复到正常范围的肿瘤标志物开始再次上升，表明残留肺癌的再生长。因此，进行了WT1疫苗接种。在第一次WT1疫苗接种后，SLX开始减少，并在第二次WT1疫苗接种后恢复并保持在正常水平（34）。WT1疫苗接种已重复约2年，没有明显的不良反应。
In patient 6, SLX, a tumor marker that had returned to normal range after the operation, began to rise again, indicating the regrowth of residual lung cancer. For this reason, WT1 vaccination was performed. SLX began to decrease after the first WT1 vaccination and returned to and stayed at normal levels after the second WT1 vaccination (34). WT1 vaccination has been repeated for ≈2 years without significant adverse effect.
在患者12中，肿瘤标志物SCC和CYFRA在WT1疫苗接种后逐渐减少（图2）。由于WT1疫苗接种被认为是有效的，因此这三个患者（患者3,6和12）在方案的三个初始方案完成后接受了重复的WT1注射。
In patient 12, the tumor markers, SCC and CYFRA, gradually decreased after the WT1 vaccination (Fig. 2). These three patients (patients 3, 6, and 12) received repeated WT1 injections after the completion of the protocol's three initial ones because WT1 vaccination was considered to be effective.

图2。
患有肺癌的患者12中肿瘤标志物（SCC）的减少。虚线表示正常范围的上限。